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Cytogenetics Specimen Requirements, Mailing Instructions and Forms

Specimen Requirements

Please follow these instructions for drawing and transporting any specimens to Sanford Clinic USD Genetics Laboratory.

PLEASE NOTE: Keep all samples at room temperature and send immediately to the Genetics Laboratory by overnight courier.
DO NOT REFRIGERATE or FREEZE. If you have any questions, please don’t hesitate to call us at 605-333-5202.

Peripheral Blood

5 cc of blood (each) drawn into both an EDTA tube and a sodium heparin tube for array CGH studies. 1-2 cc each is acceptable from infants and small children. 5 cc of blood drawn into one sodium heparin tube for chromosome and FISH studies. 1-2 cc is acceptable from infants and small children. Results are typically available 10 days after receipt. In emergencies, preliminary results are available in 4-5 days by telephone.

Solid Tissue

A tissue biopsy (2-3mm) obtained aseptically and transferred to a sterile vial containing sterile saline or tissue transport media (obtained from the Genetics Laboratory). Results are available 2 to 4 weeks after receipt.

Abortus Material

A tissue biopsy (2-3 mm) obtained aseptically, washed in sterile saline and transferred to a sterile vial containing sterile saline, tissue transfer media or maternal serum. Alcohol should be used as the disinfectant as betadine is toxic to cells. If the fetus is macerated or the parents refuse autopsy, the following samples are acceptable:

1. A 1 cm tissue sample obtained from the fetal side of the placenta, adjacent to cord insertion,
2. Periosteum overlying the sternum or fascia above the inguinal ligament, or
3. Chorionic villi. Fetal tissue can also be collected from multiple sites such as the kidneys, thymus, lungs, diaphragm, muscles, tendons, and gonads. An amniocentesis may also be performed should fetal demise be recognized before delivery. Results are available 2 to 4 weeks after receipt.

Amniotic Fluid Cells

20-30 cc of aseptically obtained amniotic fluid distributed into 2-3 sterile test tubes, 10 cc each. The tubes are to be labeled #1, #2, and #3. The initial test tube may be slightly bloody. Tubes #2 and #3 should be blood free, the latter being used for AFP determination. Results are available 2 weeks after receipt. In most cases, preliminary telephone reports are available in 8 to 10 days.

Viable cells are necessary for genetic studies, therefore, plastic syringes and conical centrifuge tubes should be used for the collection and transportation of the amniotic fluid. Do not spin the tubes before submitting.

A signed consent form from the patient MUST accompany the specimen along with a completed amniotic fluid chromosome analysis questionnaire form and requisition. Forms are supplied on request.

Bone Marrow

2-5 cc of bone marrow should be placed in a sodium heparin tube. Please fax the pathology report to the Genetics Laboratory at 605-328-2801. Results are available 2 weeks after receipt.

Samples are accepted Monday through Friday, 8 am - 5 pm. Please notify the laboratory if a specimen is to be received on a Saturday or Sunday as the laboratory has variable weekend hours.

Mailing Instructions & Forms

Shipping Instructions

1. All specimens should be labeled with two unique patient identifiers, and be accompanied by completed order forms.
2. Send specimens via courier (in Sioux Falls) or contact the laboratory for pick-up (w/in Sanford USD Medical Center).
3. Outside of Sioux Falls, specimens may be sent via O/N delivery.
4. Please confirm that specimen containers are sealed, wrapped in absorbent material, and placed in biohazard bags prior to being placed in shipping containers. Protect specimens from temperature extremes – please do NOT freeze.
5. Do not hesitate to contact laboratory personnel with any questions or concerns.

Posted Date: June 2011