SIOUX FALLS, S.D. – A June edition of the Proceedings of the National Academy of Sciences of the United States of America features the work of Sanford Research’s Kyle J. Roux, Ph.D, who developed a system to identify interactions between living cells called BioID.
Roux’s study, “Probing nuclear pore complex architecture with proximity-dependent biotinylation,” demonstrates the utility of BioID (proximity-dependent biotinylation) for application in cell research.
Through the fusion of an enzyme to a protein, BioID allows researchers to monitor the proximity and interactions between proteins in living cells, providing insight into the underlying mechanisms of disease. BioID advances the rate at which scientists can characterize proteins and their interactions, helping in the design of therapies for human application.
In this most recent study, Roux and his team used BioID to explore the organization of the nuclear pore complex, a large subcellular structure that regulates molecular transport between different compartments of the cell. They also measured the labeling distance of BioID.
“We gained a better understanding of the architecture of the nuclear pore and further demonstrated BioID as a valuable tool for exploring the organization of large protein assemblies,” said Roux. “Furthermore, determining the labeling radius of BioID allows for the rational application of this method and enables more meaningful data interpretation.”
Roux is a scientist in the Children’s Health Research Center at Sanford Research. In August, he received a five-year, $1.52 million grant from the National Institutes of Health to further develop BioID.
PNAS is the official journal of the United States National Academy of Sciences. Coverage in PNAS spans the biological, physical and social sciences.
About Sanford Research
Sanford Research is a non-profit research organization and is part of Sanford Health, an integrated health system headquartered in the Dakotas. Sanford represents the largest, rural, not-for-profit health care system in the nation with a presence in 111 communities, nine states and two countries. In 2007, a transformational gift of $400 million by Denny Sanford provided for an expansion of children’s and research initiatives, one of which was to find a cure for type 1 diabetes, and has given Sanford Research significant momentum in its goal of becoming one of the premiere research institutions in the United States and the world. Most recently, subsequent gifts of more than $200 million by Mr. Sanford have paved the way to establish Edith Sanford Breast Cancer Research and Sanford Imagenetics.
With a team of more than 200 researchers, Sanford Research comprises several research centers, including Children’s Health Research, Edith Sanford Breast Cancer, Cancer Biology, Center for Health Outcomes and Prevention and Sanford Sports Science Institute.